Fig. 2. Transitive linking yields large coherent groups.

a, We transitively grouped clonotypes at given per cent CDRH3 amino acid identities (boxed) while requiring the same heavy chain V gene. The graph shows the relationship between light chain coherence and the number of cells appearing in each group. b, Top, at 90% identity, the transitive group containing a cell with the CDRH3 sequence CIKDILPGGADSW is shown, using the data from this Article and from Phad et al. (2022)³⁶. These cells use the heavy chain V gene IGHV3-9. Each dot represents a cell, and each cluster is a computed clonotype. With the exception of three cells, all computed clonotypes use the light chain gene IGKV2-30, and cells from all six donors (d1, d2, d3, d4, d5 and d6) are present. Bottom, logo plot for CDRH3 (top) and CDRL3 (bottom) amino acid sequences in this group.