FIG. 2.

Cytokine mRNA expression in the brains of C. parvum antigen-challenged mice. Total RNA (50 μg) from control (saline injection) or C. parvum antigen-challenged mouse brains at various time points was used in the multitemplate RPA. Expression of cytokines, their receptors, and GAPDH (as an internal control) in individual mouse brain was quantified. The average ratio of cytokine or cytokine receptor to GAPDH in the control mouse was used as a baseline. There were four mice per group per time period. Asterisks, expression below detectable threshold.