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. 2022 Oct 11;94(42):14659–14665. doi: 10.1021/acs.analchem.2c02895

Figure 2.

Figure 2

BiMW-based microRNA-21-5p hybridization assay in buffer. (A) Real-time sensorgrams showing the specific interaction of the DNA probe with different microRNA-21-5p concentrations. Nonspecific microRNA-210-3p (control) was measured at a concentration of 100 nM. (B) Calibration curve in hybridization buffer (SSC 5X) (log–log display). Each signal corresponds to the mean ± SD of triplicate measurements. IN (t ∼ 500 s) and OUT (t ∼ 1500 s) arrows indicate the start and end time of the injection, respectively.