Table 4.

Experiment 2: Blood parameters of Holstein cows fed diets supplemented with isolated or commercial E. faecium as probiotics.

	Diet a			SEM	P -value
	Control	Isolated	Commercial		Diet	Control vs. others	Isolated vs. Commercial
Total proteins, g/dL	9.58	9.93	9.96	0.15	0.193	0.073	0.897
Albumin, g/dL	5.35	5.60	5.52	0.14	0.480	0.252	0.715
Globulin, g/dL	4.23	4.33	4.43	0.13	0.589	0.385	0.590
Albumin: globulin ratio	1.29	1.32	1.27	0.06	0.833	0.943	0.553
Urea-N, mg/dL	78.9	76.8	81.3	1.54	0.144	0.917	0.052
Glucose, mg/dL	74.0b	81.5a	80.3a	1.39	0.002	0.004	0.533
Cholesterol, mg/dL	172a	152b	144b	5.20	0.002	0.007	0.313
Triglycerides, mg/dL	109a	101a	97b	1.30	0.002	0.002	0.034
AST, Units/L	35.3a	30.0b	33.0a	1.27	0.023	0.021	0.012
ALT, Units/L	23.5a	22.3a	20.6b	0.82	0.038	0.049	0.016

AST, Aspartate aminotransferase; ALT, Alanine aminotransferase. Means in the same row with different letters differ (P < 0.05); SEM, standard error of the mean.

^aDiet: Control diet contained 30% berseem clover + 30% corn silage + 15% soybean meal + 25% yellow corn, without additives, or supplemented with E. faecium EGY_NRC1 (isolated to contains 1.1 × 10⁹ CFU/g) or commercial E. faecium NCIMB 11181 (isolated to contains 2 × 10¹² CFU/g) at 2 g/kg feed daily/cow.