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. 2022 Oct 19;26(6):358. doi: 10.3892/mmr.2022.12875

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Copyright: © Kim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — LPS specifically promotes the expression and secretion of LCN2 in macrophages. (A and B) After stimulation with varying (A) concentrations or (B) periods of LPS for the indicated time periods, RAW264.7 cells were isolated, and the conditioned media was collected. Western blotting and RT-PCR were performed. The top images are from the RT-PCR, and the lower images are western blots. GAPDH was used as a loading control. LPS, lipopolysaccharide; LCN2, lipocalin 2; RT-PCR, reverse transcription-PCR.