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. 2022 Jun 10;101(12):1517–1525. doi: 10.1177/00220345221100773

Figure 1.

Figure 1.

Morphology and growth characteristics of newly established ameloblastoma (AB) cell lines. (A) Histology of AB parent tumors from which the cell lines were derived. Hematoxylin and eosin stains shown for representative parent tumors AB2, AB3, and AB4; bar is 100 µM. (B) Morphology of corresponding TERT-transduced AB cell lines (AB2T, AB3T, and AB4T) cultured in conditional reprogramming (CR) medium. Photographed using an inverted light microscope at 10× magnification. (C) Growth rate of AB2T and AB4T cells cultured in different media formulations. CR–C and CR–Y are deficient in the conditioned component or the ROCK inhibitor (Y27632), respectively. Equal cell numbers were plated in duplicate and viable cell numbers counted (by flow cytometry) over 5 serial passages. Error bars represent 1 SD. #P < 0.10, *P < 0.05 (2-sided Student’s t test versus CR medium). Note the lack of significance in AB4T P4 and P5 is attributable to the small sample size (duplicates) and to the relatively larger SDs in CR P4 and P5 data points (note the log scale). KSFM, keratinocyte serum-free medium.