Figure 4.

Primary and secondary structure of hammerhead ribozymes (HHRzs) of (+) and (−) CtaHVd-LR1. The nucleotides of the catalytic core conserved in most natural hammerhead structures are in blue. The cleavage site of each ribozyme is indicated by an arrow. Nucleotides in the loops of both HHRzs potentially involved in tertiary interactions are marked by blue lines. Nucleotides in the (+) and (−) polarity are numerated considering their positions in the variant CtaHVd-LR1_3. Mutations observed in other variants are indicated in circles, with the compensatory mutations and those not modifying the proposed secondary structure of the HHRzs reported in red.