FIG. 4.
Comparison of apoptosis in M. avium subsp. paratuberculosis-infected bovine monocytes and a bovine macrophage cell line stained with Hoechst 33342. Bovine monocytes (shaded bars) and a bovine macrophage cell line (open bars) were infected with 106 CFU of live or heat-killed (hk) M. avium subsp. paratuberculosis (PTB) per ml. Some uninfected wells were treated with 500 nM staurosporine (Stauro) (positive control) or left untreated (negative control) for 6 h. The results are the mean and standard error of the mean of two experiments, using monocytes obtained from separate donors.