FIG. 5.
A M. avium subsp. paratuberculosis-infected bovine macrophage cell line is resistant to apoptosis when incubated in vitro for 24 (shaded bars) or 48 h (open bars). Monolayers of a bovine macrophage cell line (BoMac) were infected with 106 CFU of live M. avium subsp. paratuberculosis (Ptb) per ml and incubated in vitro for up to 48 h. Some uninfected wells were treated with 500 nM staurosporine (Stauro) (positive control) or left untreated (negative control). At the indicated item points, triplicate monolayers were stained with Hoechst 33342 and apoptotic cells were quantified by microscopy. The results are the mean and standard error of the mean of two experiments.