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. 2022 Oct 15;7(42):37782–37796. doi: 10.1021/acsomega.2c04784

Table 3. Thermal Denaturation Transitions of Duplex 4:7, Recorded at 260 and 235 nma.

entry [NaClO4] Tm2601 Tm2602 Tm2603 Tm2351 Tm2352 Tm2353
28 10 mM < 30     nd    
29 100 mM 45.6 ± 0.3     45.7 ± 0.3    
30* 1 M 63.2 ± 0.5     45.2 ± 0.6 59.8 ± 1.1  
31* 4.2 M 46.4 ± 3.1 59.3 ± 1.4 73.1 ± 1 49.8 ± 3.6 59.7 ± 1 73.1 ± 1.3
a

All measurements were carried out in duplicate, in the absence of Mg2+ containing [NaCl] = 10 mM and sodium phosphate (10 mM, pH 7.2). Entries with a (*) indicate instances that display increased thermal stability, compared to the canonical analogue.