Table 3. Thermal Denaturation Transitions of Duplex 4:7, Recorded at 260 and 235 nma.
| entry | [NaClO4] | Tm2601 | Tm2602 | Tm2603 | Tm2351 | Tm2352 | Tm2353 |
|---|---|---|---|---|---|---|---|
| 28 | 10 mM | < 30 | nd | ||||
| 29 | 100 mM | 45.6 ± 0.3 | 45.7 ± 0.3 | ||||
| 30* | 1 M | 63.2 ± 0.5 | 45.2 ± 0.6 | 59.8 ± 1.1 | |||
| 31* | 4.2 M | 46.4 ± 3.1 | 59.3 ± 1.4 | 73.1 ± 1 | 49.8 ± 3.6 | 59.7 ± 1 | 73.1 ± 1.3 |
All measurements were carried out in duplicate, in the absence of Mg2+ containing [NaCl] = 10 mM and sodium phosphate (10 mM, pH 7.2). Entries with a (*) indicate instances that display increased thermal stability, compared to the canonical analogue.