Table 4. Thermal Denaturation Transitions of Duplex 8:9 or 10:11, Recorded at 260 nm^a.

duplex 8:9				duplex 10:11
entry	[MgCl2]	[NaCl]	Tm	entry	[MgCl2]	[NaCl]	Tm	Tm2
32	5 mM	0.01–3.6 M	64.6 ± 1.1	40	5 mM	10 mM	33.8 ± 0.7
33		10 mM	46.1 ± 0.7	41	5 mM	0.1–2 M	59.4 ± 2.4
34		0.7–2.5 M	65.7 ± 0.8	42*	5 mM	2.5–4.7 M	76.1 ± 3.1	86.1 ± 1.1
35		3.6 M	62.5 ± 0.5	43*		0.75 M	69.6 ± 0.9
				44*		1.5 M	74.1 ± 0.3
				45*		2.5 M	48.9 ± 0.3 ↑	79.6 ± 2.1
				46*		3.5 M	46.3 ± 0.3 ↑	> 80
		[NaClO4]				[NaClO4]
36		0.1 M	56.1 ± 1.3	47	5 mM	0.1–2.5 M	34.1 ± 3.9 ↓	62.3 ± 2.2
				48	5 mM	4.7 M	23.1 ± 9.6	51.9 ± 0.6
37		1 M	59.3 ± 0.8	49*		1 M	64.7 ± 0.7
38		2.7 M	45.2 ± 1.1	50*		2.7 M	61.8 ± 0.6
39		5.4 M	21.8 ± 1.8	51*		5.4 M	50.1 ± 0.5

^aAll measurements were carried out in duplicate, in the absence of Mg. Entries with a (*) indicate instances that display increased thermal stability, compared to the canonical analogue.