Table 1.

Primer sequences containing attB sites used in the amplification and cloning of the target regions for Gateway cloning.

Primer’s Name	Sequence (5′–3′)
C-type-GW-For	GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATACGATACAA AGATGAACTATTTCCTAATGTATGTAATAAAGGATGGG
C-type-GW-Rev	GGGGACCACTTTGTACAAGAAAGCTGGGTATCAATTATATAA CTTTTAACACAGATTAT
EEVGp-GW-For	GGGGACAAGTTTGTACAAAAAAGCAGGCTTAGAATACAAAAAT GTTATTAAAAAAATGTTATTTAAA
EEVGp-GW-Rev	GGGGACCACTTTGTACAAGAAAGCTGGTATTAACAACAA TTATAATAGTTTGACTCG

The underlined 25 nucleotides are attB sites followed by four “G” residues at the 5’ end; “TA” and “A” in bold are added, respectively, on the forward and reverse primers to maintain the frame shift; and the remaining residues next to the bold nucleotide/s are derived from gene sequence.