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. 2022 Oct 11;27(20):6794. doi: 10.3390/molecules27206794

Table 5.

Process parameters and their levels for lipid extraction.

Symbol Parameters Level 1 Level 2 Level 3
A methanol:chloroform ratio (v/v) M:C 2:1 M:C 1:1 M:C 1:2
B biomass: solvent ratio (w/v) 1:10 1:30 1:60
C extraction time (min) 30 60 120