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. 2022 Oct 12;14(20):4247. doi: 10.3390/nu14204247

Figure 6.

Figure 6

Red pepper seed-induced autophagy is AMPK dependent in a cell model of OA-induced steatosis. HepG2 cells were incubated with either control or AMPK siRNA for 24 h, and then treated with RPS (0 or 200 μg/mL) for additional 24 h. (A) Experimental hypothesis. (B) Oil Red O staining. (C) Protein expression of autophagy. (D) Expression of proteins associated with AMPK/mTOR/ULK pathway. β-actin was used as a control for quantification. All data represent three independent biological replicates. Different letters are significantly different according to Duncan’s multiple range test (p < 0.05).