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. 2022 Oct 7;58(10):1411. doi: 10.3390/medicina58101411

Figure 2.

Figure 2

The effect of co-culturing with C1R, C1R-B*2704 C67S, or C1R-B*2704 cells on the NK cell-mediated cytotoxicity. (A) Western blotting analysis of the perforin expression in NK-92MI cells co-cultured with C1R, C1R-B*2704 C67S, or C1R-B*2704. An aliquot (50 μg) of extracted proteins was separated with SDS-PAGE and analyzed via Western blotting, probed for actin and perforin. (B) The ratio of perforin/actin averaged from four independent experiments in Figure 2A is plotted. (C) Analysis of NK-92MI-mediated cytotoxicity via flow cytometry. The apoptotic cells and C1R cells were stained with propidium iodide and anti-CD19 antibodies, respectively. (D) The percentages of apoptotic cells induced by NK-92MI-mediated cytotoxicity averaged from four independent experiments in Figure 2C are plotted. (E) The effect of co-culturing with C1R-B*2704 cells that have been pre-treated with THU, HUA, HUNP, THUA, or THUNP on the perforin expression of NK-92MI. (F) The ratio of perforin/actin averaged from four independent experiments in Figure 2E is plotted. The ratio of perforin/actin obtained from the control was set to one. (G). The effect of co-culturing with C1R-B*2704 in the presence of BH2 or mouse IgG on the perforin expression of NK-92MI. (H) The ratio of perforin/actin averaged from four independent experiments in Figure 2G is plotted. The ratio of perforin/actin obtained from the control was set to 100%.