TABLE 1.
Bacterial strains used in this study
| Strain | Serotype | Description | MSHAa | 16-bp deletion in invertible elementb | Source or referencec |
|---|---|---|---|---|---|
| STEC strains | |||||
| 96-7 | O157:H7 | ST1, ST2 | − | + | FIHES |
| 96-24 | O157:H7 | ST1, ST2 | − | + | FIHES |
| 98E1 | O157:H7 | ST1, ST2 | − | + | FIHES |
| 98E2 | O157:H7 | ST2 | − | + | FIHES |
| 98E3 | O157:H7 | ST1, ST2 | − | + | FIHES |
| 98E5 | O157:H7 | ST1, ST2 | − | + | FIHES |
| CL-49 | O157:H7 | ST2 | − | + | 6 |
| 86-24 | O157:H7 | ST2 | − | + | 14 |
| 467 | O157:H7 | ST2 | − | + | FCIHE |
| 468 | O157:H7 | ST2 | − | + | FCIHE |
| Sakai | O157:H7 | ST1, ST2 | − | + | 31 |
| E333 | O157:H− | ST2 | − | + | FCIHE |
| 98E6 | O157:H− | ST2 | − | + | FIHES |
| E32511 | O157:H− | ST2 | − | + | 28 |
| 97E12 | O26:H11 | ST1 | + | − | FIHES |
| 97E16 | O26:H11 | ST1 | + | − | FIHES |
| 97E2 | O111:H− | ST1 | + | − | FIHES |
| 97E22 | O111:H− | ST1 | + | − | FIHES |
| Non-ST-producing O157 strains | |||||
| FSE5 | O157:H7 | − | + | FIHES | |
| FSE13 | O157:H7 | − | + | FIHES | |
| 96-39 | O157:H45 | +* | − | FIHES | |
| 96-11 | O157:H45 | + | − | FIHES | |
| EPEC strains | |||||
| 1646-3 | O55:H− | +* | − | IMSUT | |
| 2 | O55:H6 | + | − | SSI | |
| F76193 | O55:H6 | − | − | SSI | |
| 85/91-5 | O55:H7 | +* | − | SSI | |
| 70 | O55:H7 | + | − | SSI | |
| F57076 | O55:H7 | + | − | SSI | |
| PE163 | O55:H7 | + | − | OPIPH | |
| PE182 | O55:H7 | + | − | OPIPH | |
| 807-257 | O55:H7 | + | − | OPIPH | |
| F43341 | O111:H12 | + | − | SSI | |
| 118 | O111:H12 | + | − | SSI | |
| TEC1101 | O55:H10 | − | ND | TMRLPH | |
| TEC1117 | O55:H10 | − | ND | TMRLPH | |
| E98445 | O55:H10 | − | ND | TMRLPH | |
| ETEC strains | |||||
| BK005 | − | ND | RIMDOU | ||
| CH5667 | + | − | CSTRI | ||
| CH28991 | +* | − | CSTRI | ||
| O22 | + | − | This laboratory | ||
| O29 | − | ND | This laboratory | ||
| EAggEC strains | |||||
| TL100 | − | ND | 51 | ||
| 17-2 | + | ND | 46 | ||
| E2 | − | − | 47 | ||
| 253-1-1 | + | − | RIMCJ | ||
| 452-1-1 | − | ND | RIMCJ | ||
| Laboratory strain (DH5α) | + | − | 40 |
Determined by hemagglutination of guinea pig erythrocytes with or without 2% d-mannose. +, hemagglutinating strain after serial passages; +*, originally hemagglutinating strain (at start of this study).
ND, not determined; no amplification in PCR with primers used in this study.
FIHES, Fukuoka Institute of Health and Environmental Sciences; FCIHE, Fukuoka City Institute for Hygiene and Environment; IMSUT, The Institute of Medical Science, The University of Tokyo; SSI, The State Serum Institute, Copenhagen, Denmark; OPIPH, The Osaka Prefectural Institute of Public Health; TMRLPH, The Tokyo Metropolitan Research Laboratory of Public Health; RIMDOU, The Research Institute for Microbial Diseases, Osaka University; CSTRI, The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan; RIMCJ, The Research Institute, International Medical Center of Japan.