Figure 3.

Specificity determining the positions in a multiple alignment of Paramecium CaMs. The Paramecium subgroup of the protein sequences from orthogroup OG0001068 were aligned using MAFFT [126] and the resulting file uploaded in JDET [129]. Specificity-determining positions were calculated using the S3DET algorithm [129]. The figure shows a partial alignment of the output window. According to this analysis, uploaded CaM sequences were divided in five specificity subgroups, which are highlighted in red, blue, green, orange, and yellow boxes in the leftmost column of the window. This column corresponded to the identification of the proteins, but due to space restrictions, only the species name, accession number, and size of the corresponding proteins were written. The green circles mark the alignment positions that are SDPs allowing discrimination among groups.