Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 26;19:262. doi: 10.1186/s12974-022-02626-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 9 — GSK872 and Nec-1 inhibited RIP1/RIP3/MLKL pathway activation induced by glutamate excitotoxicity. a Expression levels of RIP1, RIP3, MLKL and p-MLKL (phospho S345) in R28 cells after exposure to 10 mM glutamate for 24 h with or without the addition of GSK872 or Nec-1. b Intravitreal administration of GSK872 or Nec-1 downregulated protein levels of RIP1, RIP3 and p-MLKL (phospho S345) in NMDA-damaged eyes. c Representative immunofluorescence microphotographs of retinal sections stained with RBPMS (green), RIP1 (red), and DAPI (blue) at 5 days post GSK872 and Nec-1 injection. Scale bar = 50 μm. d Representative immunofluorescence microphotographs of retinal sections stained with RBPMS (green), RIP3 (red), and DAPI (blue) at 5 days post GSK872 and Nec-1 injection. Scale bar = 50 μm. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; CTL: the control group; Glu: glutamate. The results were recorded as mean ± SD from at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control or saline group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus Glu + DMSO group or NMDA + DMSO group. ns: not significant