Thioglycoside synthesis with the different HorGH1 mutants.

Entry	Sugar donor	Enzyme variant	Molar conversion [%]
1	1a, X = pNP in β	WT	0
2	1a, X = pNP in β	M299R	0
3	1a, X = pNP in β	E166A	23
4	1a, X = pNP in β	M299R/E166A	26
5	1b, X = F in α	E354G	0
6	1b, X = F in α	M299R/E354G	0
7	1b, X = F in α	E166A/E354G	61
8	1b, X = F in α	M299R/E166A/E354G	92