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. 2022 Sep 30;12(10):928. doi: 10.3390/metabo12100928

Table 2.

The effect of intensive fat mass loss and fat mass regain on free fatty acid (FFA) and oxylipin precursor profile in the diet group of physique study participants.

Annotation and Adduct Information PRE-MID (Time) PRE-POST (Time)
Metabolite Subclass and ID Matching ID Estimate Standard Error FDR Estimate Standard Error FDR
Very-long-chain Saturated Fatty acids (VLCSFA)
Lignoceric Acid [M-H + Acetate] 546323 3.99 1.09 1.64 × 10 −3 0.21 0.39 8.88 × 10−1
Lignoceric Acid [M-H] 544841 3.33 0.72 9.39 × 10 −5 −0.1 0.28 9.22 × 10−1
Lignoceric Acid [M-H] 546284 4.42 1.30 3.17 × 10 −3 0.21 0.43 9.02 × 10−1
Tricosenoic Acid [M-H] 539371 4.14 0.83 2.75 × 10 −5 −0.46 0.26 5.04 × 10−1
Tricosenoic Acid [M-H] 542617 2.73 0.96 1.32 × 10 −2 −0.57 0.45 6.34 × 10−1
Tricosanoic Acid [M-H + Acetate] 542670 2.19 0.47 9.23 × 10 −5 −0.23 0.31 8.40 × 10−1
Tricosanoic Acid [M-H] 542623 2.27 0.48 6.24 × 10 −5 −0.17 0.34 9.00 × 10−1
Tricosanoic Acid [M-H] 544047 2.15 0.53 6.18 × 10 −4 −0.10 0.34 9.38 × 10−1
Behenic Acid [M-H + Acetate] 542079 3.02 0.88 2.84 × 10 −3 −0.60 0.41 5.74 × 10−1
Behenic Acid [M-H] 540593 2.31 0.41 1.79 × 10 −6 0.06 0.27 9.69 × 10−1
Behenic Acid [M-H] 542037 3.2 0.79 6.18 × 10 −4 −0.28 0.39 8.40 × 10−1
Behenic Acid [M-H] 539090 1.51 0.49 7.22 × 10 −3 0.85 0.80 7.02 × 10−1
Long-chain Saturated Fatty acids (LCSFA)
Stearic Acid [M-H + Acetate] 534421 −1.14 0.35 4.95 × 10 −3 0.46 0.59 8.40 × 10−1
Stearic Acid [M-H] 533317 −1.21 0.46 2.42 × 10 −2 −0.91 0.46 4.09 × 10−1
Heptadecanoic Acid† [M-H + Acetate] * 534900 −1.98 0.50 6.67 × 10 −4 −1.36 0.63 3.93 × 10−1
Heptadecanoic Acid† [M-H + Acetate] * 534128 −1.64 0.42 8.93 × 10 −4 0.11 0.55 9.69 × 10−1
Heptadecanoic Acid† [M-H] 532380 −1.29 0.40 4.51 × 10 −3 −0.02 0.42 9.86 × 10−1
Heptadecanoic Acid† [M-H] * 534537 −1.44 0.47 7.30 × 10 −3 −0.61 0.49 6.34 × 10−1
Heptadecaenoic Acid† [M-H + Acetate] * 529424 −1.86 0.41 1.20 × 10 −4 −0.49 0.57 7.96 × 10−1
Heptadecaenoic Acid† [M-H + Acetate] * 532672 −1.54 0.50 6.57 × 10 −3 −0.34 0.66 8.90 × 10−1
Heptadecaenoic Acid† [M-H + Acetate] 531646 6.21 2.72 4.80 × 10 −2 0.73 0.59 6.34 × 10−1
Heptadecaenoic Acid† [M-H] 529369 −1.83 0.45 6.39 × 10 −4 −0.12 0.55 9.61 × 10−1
Heptadecaenoic Acid† [M-H] 531845 −1.16 0.37 6.36 × 10 −3 −0.10 0.45 9.61 × 10−1
Palmitic Acid [M-H + Acetate] 531306 −1.63 0.41 8.72 × 10 −4 −0.36 0.48 8.40 × 10−1
Palmitic Acid [M-H + Acetate] 534889 −0.72 0.30 3.87 × 10 −2 0.09 0.34 9.46 × 10−1
Palmitic Acid† [M-H + Acetate] * 531996 −1.38 0.42 4.37 × 10 −3 −0.04 0.53 9.86 × 10−1
Palmitic Acid [M-H] 533569 −0.76 0.32 3.81 × 10 −2 0.46 0.42 6.89 × 10−1
Pentadecanoic Acid [M-H] 530326 −1.45 0.45 5.19 × 10 −3 −0.33 0.47 8.48 × 10−1
Pentadecanoic Acid [M-H] 528566 −1.15 0.46 2.95 × 10 −2 −0.22 0.51 9.03 × 10−1
Pentadecanoic Acid† [M-H + Acetate] * 531478 −2.07 0.45 9.24 × 10 −5 −0.27 0.72 9.22 × 10−1
Myristic Acid [M-H + Acetate] 524395 −1.63 0.53 6.50 × 10 −3 −0.34 0.75 9.03 × 10−1
Myristic Acid [M-H + Acetate] 522387 −1.74 0.50 2.45 × 10 −3 −0.05 0.58 9.86 × 10−1
Myristic Acid [M-H] * 524668 −1.55 0.48 4.97 × 10 −3 −0.33 0.63 8.90 × 10−1
Myristic Acid [M-H] 522346 −1.64 0.50 4.48 × 10 −3 −0.2 0.56 9.22 × 10−1
Very-long-chain Monounsaturated Fatty acids
Tricosenoic Acid [M-H] 539371 4.14 0.83 2.75 × 10 −5 −0.46 0.26 5.04 × 10−1
Tricosenoic Acid [M-H] 542617 2.73 0.96 1.32 × 10 −2 −0.57 0.45 6.34 × 10−1
Tricosanoic Acid [M-H + Acetate] 542670 2.19 0.47 9.23 × 10 −5 −0.23 0.31 8.40 × 10−1
Omega-3 Fatty Acids
Docosahexaenoic Acid (DHA) [M-H + Acetate] 529467 −1.01 0.40 2.71 × 10 −2 0.02 0.38 9.86 × 10−1
Docosahexaenoic Acid (DHA) [M-H] 525743 −1.03 0.32 5.40 × 10 −3 0.32 0.37 7.93 × 10−1
\ Docosatrienoic Acid [M-H] 532415 −1.5 0.37 5.70 × 10 −4 0.35 0.46 8.40 × 10−1
Eicosapentaenoic Acid (EPA) [M-H + Acetate] 523035 1.34 0.35 1.08 × 10 −3 0.13 0.38 9.24 × 10−1
Eicosapentaenoic Acid (EPA) [M-H] 523009 1.26 0.36 2.28 × 10 −3 0.02 0.38 9.86 × 10−1
Stearidonic Acid [M-H] 488156 −3.39 1.35 2.84 × 10 −2 −2.48 1.28 4.09 × 10−1
α-Linolenic Acid [M-H] 523928 −1.15 0.33 2.50 × 10 −3 0.49 0.53 7.76 × 10−1
α-Linolenic Acid [M-H] 521670 −1.71 0.52 4.51 × 10 −3 −0.42 0.59 8.48 × 10−1
Omega-6 Fatty Acids
Docosadienoic Acid [M-H] 534554 −1.29 0.34 1.18 × 10 −3 0.32 0.39 8.06 × 10−1
Docosadienoic Acid [M-H] 536158 −1.52 0.47 5.13 × 10 −3 −0.95 0.51 4.53 × 10−1
Eicosadienoic Acid [M-H] 531992 −1,50 0.32 9.39 × 10 −5 0.23 0.37 8.69 × 10−1
Linoleic Acid [M-H] 526174 −1.39 0.38 1.64 × 10 −3 0.04 0.45 9.86 × 10−1
Dihomo- γ -linolenic [M-H] 530664 −1.71 0.28 8.71× 10 −8 0.43 0.38 6.79 × 10−1
Dihomo- γ -linolenic [M-H] 529407 −1.61 0.35 1.02 × 10 −4 0.20 0.40 8.95 × 10−1
Arachidonic Acid [M-H] 529143 −1.42 0.29 4.21 × 10 −5 0.25 0.49 9.00 × 10−1
Arachidonic Acid [M-H + Acetate] * 531873 −1.30 0.36 1.64 × 10 −3 0.20 0.38 8.88 × 10−1
Adrenic Acid [M-H] 528116 −1.45 0.33 1.82 × 10 −4 1.10 0.51 3.93 × 10−1
Adrenic Acid [M-H] 532126 −1.83 0.43 2.69 × 10 −4 −0.26 0.50 8.89 × 10−1
Adrenic Acid [M-H] 530967 −1.58 0.39 5.70 × 10 −4 0.40 0.46 7.93 × 10−1
Omega-7 Fatty Acids
Palmitoleic Acid [M-H + Acetate] 526018 −1.35 0.36 1.27 × 10 −3 0.32 0.36 7.93 × 10−1
Palmitoleic Acid [M-H + Acetate] 529152 −1.64 0.62 2.09 × 10 −2 −1.13 0.63 4.96 × 10−1
Palmitoleic Acid [M-H] 531843 −2.12 0.39 3.23 × 10 −6 −0.18 0.43 9.10 × 10−1
Palmitoleic Acid [M-H] 529701 −2.21 0.41 5.66 × 10 −6 −0.31 0.36 7.96 × 10−1
Palmitoleic Acid [M-H] 526135 −1.37 0.34 6.18 × 10 −4 0.25 0.32 8.40 × 10−1
Omega-9 Fatty Acids
Nervonic Acid [M-H] 544451 2.01 0.64 5.89 × 10 −3 −0.71 0.43 5.50 × 10−1
Nervonic Acid [M-H] 539105 3.15 0.85 1.43 × 10 −3 −0.02 0.38 9.86 × 10−1
Docosaenoic Acid† (Erucic Acid) [M-H] * 535834 3.17 0.76 3.80 × 10 −4 0.21 0.50 9.10 × 10−1
Eicosatrienoic Acid† [M-H + Acetate] * 534431 −1.54 0.44 2.29 × 10 −3 0.30 0.56 8.88 × 10−1
Eicosenoic Acid [M-H] 534395 −1.36 0.38 1.87 × 10 −3 −0.16 0.38 9.03 × 10−1
Eicosenoic Acid [M-H] 531994 −1.35 0.38 2.30 × 10 −3 0.35 0.55 8.69 × 10−1
Eicosenoic Acid [M-H] 539066 −1.46 0.45 4.51 × 10 −3 −0.12 0.44 9.46 × 10−1
Oleic Acid [M-H + Acetate] 531868 −0.94 0.36 2.42 × 10 −2 0.57 0.36 5.50 × 10−1
Oleic Acid [M-H] 535786 −1.37 0.39 2.44 × 10 −3 0.09 0.47 9.69 × 10−1
Oleic Acid [M-H] 534665 −1.4 0.44 5.48 × 10 −3 0.4 0.59 8.54 × 10−1
Oleic Acid [M-H] 531847 −0.96 0.39 3.00 × 10 −2 0.19 0.34 8.88 × 10−1

FFA = Free Fatty Acid. EIC = Eicosanoid. FDR = False Discovery Rate. FDR < 0.05 (i.e., <5 × 10−2) are bolded in the table. Results are derived from 4 standard deviation from mean quality-controlled data. Table is ordered based on metabolite feature subclasses. Statistical significance was calculated using Generalized estimation equations (~Time + age). Significance threshold was set to FDR < 0.05, and all significant metabolite features from within diet group analysis and among specified subclasses are depicted in Table 2. Analysis was adjusted with age as covariate. * Denotes metabolite features that were also significant (FDR < 0.05) and modulated in the same direction in the Control group analysis.