Table 2.
Antioxidant activity of the hydroethanolic extract of the aerial parts of Sideritis raeseri subsp. raeseri and its fractions.
| Sample | Concentration [µg/mL] |
β-Carotene [% Inhibition] |
DPPH [% Inhibition] |
ABTS [% Inhibition] |
FRAP [µmol Fe (II)/g] |
||||
|---|---|---|---|---|---|---|---|---|---|
| Hydroethanolic extract | 100 200 500 |
30.12 ± 0.39 a 33.77 ± 1.26 b 38.33 ± 0.78 c |
d | 8.26 ± 0.79 a 17.51 ± 0.97 b 45.22 ± 0.53 c |
b | 8.14 ± 0.59 a 18.32 ± 0.69 b 34.37 ± 0.37 c |
c | 140.72 ± 5.18 a 167.70 ± 5.71 b 389.79 ± 3.80 c |
c |
| Petroleum ether fraction | 100 200 500 |
19.69 ± 1.48 a 22.69 ± 1.03 a 36.51 ± 1.48 b |
c | 2.67 ± 0.32 a 4.99 ± 0.70 b 6.96 ± 0.32 c |
a | 1.75 ± 0.22 a 3.05 ± 0.44 b 6.54 ± 0.59 c |
a | 32.38 ± 3.13 a 52.30 ± 3.13 b 74.72 ± 4.00 c |
a |
| Ethyl acetate fraction | 100 200 500 |
28.55 ± 1.17 a 34.94 ± 0.60 b 41.07 ± 0.78 c |
d | 12.31 ± 0.48 a 26.86 ± 0.91 b 69.41 ± 0.63 c |
d | 22.39 ± 0.51 a 37.37 ± 0.47 b 57.44 ± 0.80 c |
e | 132.84 ± 3.74 a 353.67 ± 5.03 b 501.87 ± 3.80 c |
c |
| Butanol fraction | 100 200 500 |
2.50 ± 2.10 a 7.09 ± 1.20 b 13.21 ± 1.50 c |
a | 13.71 ± 0.38 a 45.99 ± 0.53 b 91.60 ± 0.78 c |
e | 13.96 ± 0.47 a 29.96 ± 0.37 b 50.36 ± 0.37 c |
d | 137.82 ± 3.29 a 354.50 ± 6.93 b 603.99 ± 5.18 c |
c |
| Water fraction | 100 200 500 |
7.93 ± 0.64 a 8.90 ± 1.93 a 17.39 ± 0.83 b |
b | 10.48 ± 0.75 a 15.40 ± 0.97 b 45.04 ± 0.63 c |
c | 5.04 ± 0.67 a 13.33 ± 0.25 b 25.40 ± 0.44 c |
b | 61.44 ± 4.37 a 129.10 ± 4.98 b 278.95 ± 5.18 c |
b |
| BHA | 100 | 57.70 ± 1.91 | e | 43.33 ± 0.87 | g | 64.95 ± 0.63 | g | 572.85 ± 5.71 | e |
| BHT | 100 | 56.29 ± 1.44 | e | 34.31 ± 0.43 | f | 55.11 ± 0.44 | f | 413.03 ± 3.13 | d |
| Ascorbic acid | 100 | 2.99 ± 2.13 | a | 91.65 ± 0.21 | h | 55.70 ± 0.69 | f | 576.17 ± 7.61 | e |
Different letters (a–c) within the same cells indicate a significant difference (p < 0.05) in activity between different tested concentrations while different letters (a–h) in the same columns indicate a significant difference in activity between extract/fractions and standards at the concentration of 100 μg/mL according to the post hoc Tukey’s test.