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. 2022 Oct 21;14(10):2313. doi: 10.3390/v14102313

Figure 3.

Figure 3

p53 transcriptional activity is not essential for inhibition of HBV replication. (A,B) HepG2-NTCP and Hep3B-NTCP cells were transfected with either an empty vector or p53 expression plasmid for 24 h and either mock-infected or infected with HBV at 50 GEQ/cell for 4 days in the presence or absence of pifithrin-alpha (PFT-α), followed by Western blotting. (B) Levels of HBeAg released from the cells prepared in (A) were measured by ELISA (n = 4). (D) The levels of HBeAg released from the cells prepared in (C) were measured by ELISA (n = 5).