Figure 5.

Characteristics of POWV infection in hNSC-derived neuron/astrocyte co-cultures. hNSC-derived neuron/astrocyte co-cultures were infected with 1.0 MOI POWV and cells fixed at 72 h post-infection. Neurons were stained using anti-MAP2 (green) antibodies, astrocytes were stained using anti-GFAP (blue) antibodies and POWV was stained using anti-E protein antibodies (red). (a) 20× optical image. (b) 63× optical image. (c) 63× optical image with 5× digital zoom. (d–f) Three-dimensional rendering of neurons and virus using IMARIS, focusing on viral aberrations. Average size of the aberrations and artifacts were compared by measuring the size of the MAP2 signal and (g) using a t-test with Welch’s correction and (h) size distribution was calculated in a histogram. **** p ≤ 0.0001.