Table 1.
Technical Fluorescence Parameters | |
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Ft | fluorescence at time t after onset of actinic illumination |
FO ≅ F20μs | minimal fluorescence, when all PSII RCs are open |
FL ≡ F150μs | fluorescence intensity at the L-step (150 μs) of OJIP |
FK ≡ F300μs | fluorescence intensity at the K-step (300 μs) of OJIP |
FJ ≡ F2ms | fluorescence intensity at the J-step (2 ms) of OJIP |
FI ≡ F30ms | fluorescence intensity at the I-step (30 ms) of OJIP |
FP (= M) | maximal fluorescence, at the peak P of OJIP |
Fv ≡ Ft − FO | variable fluorescence at time t |
Vt ≡ (Ft − FO)/(FM − FO) | relative variable fluorescence at time t |
VJ = (FJ − FO)/(FM − FO) | relative variable fluorescence at the J-step |
VI = (FI − FO)/(FM − FO) | relative variable fluorescence at the I-step |
Quantum efficiencies or flux ratios | |
φPo = PHI(P0) = TR0/ABS = 1 − FO/FM | maximum quantum yield for primary photochemistry |
ψΕo = PSI0 = ET0/TR0 = (1 − VJ) | probability that an electron moves further than QA− |
φEo = PHI(E0) = ET0/ABS = (1 − FO/FM) (1 − VJ) | quantum yield for electron transport (ET) |
Phenomenological energy fluxes (per excited leaf cross-section-CS) | |
ABS/CS = Chl/CS | absorption flux per CS |
TR0/CS = φPo·(ABS/CS) | trapped energy flux per CS |
ET0/CS = φPo·ψΕo·(ABS/CS) | electron transport flux per CS |
Density of reaction center (QA-reducing PSII reaction center–RC) | |
RC/CS = φPo·(VJ/M0)·(ABS/CS) | density of QA-reducing PSII RCs per CS |
QA-reducing centers = (RC/RCreference)·(ABS/ABSreference) = [(RC/CS)treatment/(RC/CS)control]·[(ABS/CS)treatment/(ABS/CS)control] |
fraction of QA-reducing PSII RCs |
RJ = (ψΕo(control) − ψΕo(treatment))/ψΕo(control) = (VJ(treatment) − VJ(control))/(1 − VJ(control)) |
number of PSII RCs with QB-site filled by PSII inhibitor |
Performance indexes | |
≡ | performance index for energy conservation from photons absorbed by PSII to the reduction of intersystem electron acceptors |
Phenomenological energy fluxes (per excited leaf cross-section-CS) | |
driving force on absorption basis |
1 Subscript “0” (or “o” when written after another subscript) indicates that the parameter refers to the onset of illumination, when all RCs are assumed to be open.