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. 2022 Oct 21;11(20):2797. doi: 10.3390/plants11202797

Table 1.

Formulae and explanation of the technical data of the OJIP curves and the selected JIP-test parameters used in this study 1.

Technical Fluorescence Parameters
Ft fluorescence at time t after onset of actinic illumination
FO ≅ F20μs minimal fluorescence, when all PSII RCs are open
FL ≡ F150μs fluorescence intensity at the L-step (150 μs) of OJIP
FK ≡ F300μs fluorescence intensity at the K-step (300 μs) of OJIP
FJ ≡ F2ms fluorescence intensity at the J-step (2 ms) of OJIP
FI ≡ F30ms fluorescence intensity at the I-step (30 ms) of OJIP
FP (= M) maximal fluorescence, at the peak P of OJIP
Fv ≡ Ft − FO variable fluorescence at time t
Vt ≡ (Ft − FO)/(FM − FO) relative variable fluorescence at time t
VJ = (FJ − FO)/(FM − FO) relative variable fluorescence at the J-step
VI = (FI − FO)/(FM − FO) relative variable fluorescence at the I-step
Quantum efficiencies or flux ratios
φPo = PHI(P0) = TR0/ABS = 1 − FO/FM maximum quantum yield for primary photochemistry
ψΕo = PSI0 = ET0/TR0 = (1 − VJ) probability that an electron moves further than QA
φEo = PHI(E0) = ET0/ABS = (1 − FO/FM) (1 − VJ) quantum yield for electron transport (ET)
Phenomenological energy fluxes (per excited leaf cross-section-CS)
ABS/CS = Chl/CS absorption flux per CS
TR0/CS = φPo·(ABS/CS) trapped energy flux per CS
ET0/CS = φPo·ψΕo·(ABS/CS) electron transport flux per CS
Density of reaction center (QA-reducing PSII reaction center–RC)
RC/CS = φPo·(VJ/M0)·(ABS/CS) density of QA-reducing PSII RCs per CS
QA-reducing centers = (RC/RCreference)·(ABS/ABSreference)
= [(RC/CS)treatment/(RC/CS)control]·[(ABS/CS)treatment/(ABS/CS)control]
fraction of QA-reducing PSII RCs
RJ = (ψΕo(control) − ψΕo(treatment))/ψΕo(control)
= (VJ(treatment) − VJ(control))/(1 − VJ(control))
number of PSII RCs with QB-site filled by PSII inhibitor
Performance indexes
PIABSγRC1γRC·φPo1φPo·ΨEo1ΨEo performance index for energy conservation from photons absorbed by PSII to the reduction of intersystem electron acceptors
Phenomenological energy fluxes (per excited leaf cross-section-CS)
DFABS=logPIABS=logRCABS+log(ϕPo1ϕPo)+log(ψEo1ψEo) driving force on absorption basis

1 Subscript “0” (or “o” when written after another subscript) indicates that the parameter refers to the onset of illumination, when all RCs are assumed to be open.