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. 2022 Oct 15;15(10):1272. doi: 10.3390/ph15101272

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determination of the K_D value of the binding of [¹⁸F]11 towards rat PDE2A in vitro. Quantitative autoradiography has been performed by incubating 12 µm cryosections of one female SPRD rat (A) and one male Wistar-Han rat (B) with 6.8 nM [¹⁸F]11 alone or with different concentrations of non-labeled 11. By non-linear analysis of the homologous inhibition curve obtained for the percentage of specific binding of [¹⁸F]11 in caudate putamen (GraphPad Prism 9.1.2; Parameters: Nonlinear regression–One site–Homologous), an apparent K_D value of 0.24 nM (SPRD: 0.38 nM, Wistar: 0.10 nM) and an apparent B_max value of 16 pmol/mg protein (SPRD: 14 pmol/mg protein, Wistar: 18 pmol/mg protein) has been estimated.