Table 3.
The Differential Protocol Parameters Included in the Surrogate Factors for LMM Analyses
| DT_Factor1 | Fish Strain | Dechorionation | Exposure Volume | Exposure Scenario | Measure Effect at Time | |
|---|---|---|---|---|---|---|
| Lab-A | Reference | AB Tg (Cmlc2: copGFP) | No | Larger (1000) | Static renewal | Earlier (96 hpf) |
| Lab-B | Comparison | 5D Tropical | Yes | Smaller (100/200) | Static | Later (120 hpf) |
| Lab-C | ||||||
| NT_Factor1 | Fish strain | Dechorionation | Exposure at time | |||
| Lab-A | Reference | AB wild-type | No | Later (72 hpf) | ||
| Lab-B | Comparison | 5D Tropical | Yes | Earlier (6 hpf) | ||
| Lab-C | ||||||
| NT_Factor2 | Exposure volume | No. of L/D cycles | Duration of testing | |||
| Lab-A | Reference | Larger (200) | 2 L/D | Longer (40/30 min) | ||
| Lab-C | ||||||
| Lab-B | Comparison | Smaller (100) | 3 L/D | Shorter (18 min) | ||
Abbreviations: DT, devtox; NT, neurotox; hpf, hour post fertilization.