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. 2022 Oct 14;5(5):81. doi: 10.3390/mps5050081

Figure 1.

Figure 1

Higher concentration of reduced nicotinamide adenine dinucleotide (NADH) in alanine aminotransferase (ALT) reagent confers stability of standard curve. ALT reagent with low NADH concentration (0.12 mM, black), intermediate NADH concentration (0.75 mM, blue), and high NADH concentration (1.5 mM, red). (A) First run. Left, curve with Log 2 scale on X-axis allowing detailed view of lower ALT values; right, linear scale on X-axis demonstrating linear correlation of values with high NADH ALT reagent. (B) Second run. Left, curve with Log 2 scale on X-axis allowing detailed view of lower ALT values; right, linear scale on X-axis demonstrating linear correlation of values with high NADH ALT reagent. (C) Third run. Left, curve with Log 2 scale on X-axis allowing detailed view of lower ALT values; right, linear scale on X-axis demonstrating linear correlation of values with high NADH ALT reagent. Vmax, maximum reaction velocity.