Table 2.

Acute toxicity of biosynthesized ZnO NPs against larvae and pupae of A. subpictus and C. quinquefasciatus on 24-h exposure

Species	Larvae instars	LC50 (mg/L)	95 % confidence limit		LC90 (mg/L)	95 % confidence limit		r2	χ2 (d.f. = 4)
			Lower	Upper		Lower	Upper
A. subpictus	I	1.70	0.75	2.37	6.13	5.55	6.92	0.910	1.657
	II	1.66	0.40	2.52	7.64	6.86	8.76	0.913	2.650
	III	1.93	2.55	3.61	8.63	6.91	13.92	0.850	5.424
	IV	2.48	1.27	3.32	9.45	8.43	11.00	0.876	4.795
	Pupa	3.63	0.70	5.07	9.78	7.86	15.13	0.886	6.880
C. quinquefasciatus	I	1.95	0.90	2.68	7.25	6.56	8.23	0.978	4.466
	II	2.63	1.79	3.25	7.73	7.03	8.69	0.974	1.103
	III	2.90	2.06	3.53	8.30	7.55	9.34	0.937	1.095
	IV	4.32	3.61	4.92	10.51	9.50	11.99	0.983	2.117
	Pupa	4.61	3.85	5.24	11.46	10.24	13.31	0.980	1.991

No mortality was observed in the control, significant at P < 0.05 level. Mean value of three replicates

LC₅₀ lethal concentration that kills 50 % of the exposed organisms, LC₉₀ lethal concentration that kills 90% of the exposed organisms, r² regression coefficient, χ² chi-square value d.f. degrees of freedom