Table 3.
Selected genes used for real-time qPCR studies related to protective mechanisms and/or oxidative stress control, primer sequences and amplicon size (bp).
| Gene Symbol | Gene Description | Primer Sequence (5′–3′) |
Amplicon Size (bp) |
|---|---|---|---|
| HSP70 | Stromal 70 kDa heat shock-related protein, chloroplastic | F: GGGAAGCAATTGACACCAAG | 150 |
| R: AGCCACCAGATACTGCATCC | |||
| ELIP | Chloroplast early light-induced protein | F: GCCATGATAGGGTTTGTTGC | 101 |
| R: GTCCCAATGAACCATTGCAG | |||
| Chape20 | Chloroplast 20 kDa chaperonin | F: GTTAAAGCTGCCGCTGTTG | 150 |
| R: CTCACCTCCTTGAGGTTTCG | |||
| Chape60 | Mitochondria chaperonin CPN60 | F: GGATAGTGAAGCCCTTGC | 80 |
| R: CCCAGGAGCTTTTATTGCAC | |||
| CAT | Catalase isozyme 1 | F: CTACTTCCCCTCGCGGTAT | 150 |
| R: CTGTCTGGTGCAAATGAACG | |||
| CuSOD1 | Superoxide dismutase [Cu-Zn] | F: CCCTTGGAGACACAACGAAT | 141 |
| R: GGCAGTACCATCTTGACCA | |||
| CuSOD2 | Superoxide dismutase [Cu-Zn] | F: GGGGCTCTATCCAATTCCTC | 150 |
| R: GGTTAAAATGAGGCCCAGTG | |||
| APXCyt | Cytosol ascorbate peroxidase | F: TCTGGATTTGAGGGACCTTG | 108 |
| R: GTCAGATGGAAGCCGGATAA | |||
| APXChl | Chloroplast ascorbate peroxidase | F: CACCTGCTGCTCATTTACG | 100 |
| R: GACCTTCCCAATGTGTGTG | |||
| APXt+s | Stromatic ascorbate peroxidase (sAPX) mRNA | F: AGGGCAGAATATGAAGGATTGG | 112 |
| R: CCAAGCAAGGATGTCAAAATAGCC | |||
| VDE2 | Violaxanthin de-epoxidase | F: GGGTTCAAAATGCACAAGACTG | 86 |
| R: CCCTCTTTTACCTCAGGCATTG |