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. 2022 Sep 20;10(10):1571. doi: 10.3390/vaccines10101571

Figure 1.

Figure 1

Heat map of CD4+ T cell epitope responses to anthrax PA domain I–IV peptides in human donors. Heat map representation of the epitope mapping results observed for positive CD4+ T cell IFNγ ELIspot responses in the human donor cohorts, comprising a total of 9 donors in the cutaneous anthrax (Kayseri) group and 10 donors in the AVP vaccinees (UK) group. Peptides were considered positive for the carriage of a CD4+ T cell epitope if the response was >50 SFC/106 PBMCs and 2SD above negative control, and the stimulation index (peptide response/negative control response) value was ≥1.5. The domains were defined as described previously; domain 1 = PA 1–20 to PA 241–260; domain 2 = PA 251–270 to PA 471–490; domain 3 = PA 491–510 to PA 581–600; domain 4 = PA 591–610 to PA 716–735, with some peptides overlapping the boundaries between domains) [38]. The colour bar at the right indicates the percentage of donors responding to a given epitope, with shading from white (0%) to dark blue (50%).