Table 1.
The PA peptides, identified in transgenic mouse strains and human cohorts, show relatively broad binding to common HLA-DR and HLA-DQ alleles.
| PA Peptide Sequence | HLA Transgenic Strain Responding to Epitope after PA Immunisation | Human Cohort Responding to Epitope (>20% Cohort Responding) | Relative Binding of HLA Class II | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| DR1 | DR3 | DR4 | DR7 | DR11 | DR13 | DR15 | DQ6 | DQ8 | |||
| 21GYYFSDLNFQAPMVVTSSTT40 | - | Vaccinee, Infected | 23 | 60 | 0.3 | 22 | 53 | >1908 | 118 | ND | ND |
| 31APMVVTSSTTGDLSIPSSEL50 | DR4 | - | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 41GDLSIPSSELENIPSENQYF60 | DR1 | Infected | 3312 | >728 | 46 | 424 | >1288 | >1288 | 134 | ND | ND |
| 51ENIPSENQYFQSAIWSGFIK70 | DR4 | Infected | 3 | 600 | 1 | 2 | 2 | >2733 | 55 | ND | ND |
| 61QSAIWSGFIKVKKSDEYTFA80 | - | Infected | 617 | 12 | 650 | 89 | 14 | 4 | 1 | ND | ND |
| 71VKKSDEYTFATSADNHVTMW90 | DR4 | Infected | 11 | 8 | 1 | 2 | 118 | >2733 | 45 | ND | ND |
| 91VDDQEVINKASNSNKIRLEK110 | - | Infected | 1333 | 283 | 992 | 36 | >1357 | 245 | 164 | ND | ND |
| 121QRENPTEKGLDFKLYWTDSQ140 | DR4 | Infected | >2563 | 800 | 6 | 1549 | >1357 | >2733 | 119 | ND | ND |
| 141NKKEVISSDNLQLPELKQKS160 | DQ8 | Infected | 131 | 26 | 48 | 28 | 849 | >2733 | 1 | >3054 | >166 |
| 148SDNLQLPELKQKSSNSRKKR167 | - | Infected | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 151LQLPELKQKSSNSRKKRSTS170 | DQ8 | Infected | >6667 | >667 | >1788 | >1543 | 701 | 177 | >511 | >3054 | >166 |
| 161SNSRKKRSTSAGPTVPDRDN180 | - | Infected | >6667 | >667 | >1788 | >1543 | >1336 | >1908 | >511 | ND | ND |
| 168STSAGPTVPDRDNDGIPDSL187 | - | Infected | >6667 | >667 | >1788 | >1543 | >1336 | >1908 | >511 | ND | ND |
| 171AGPTVPDRDNDGIPDSLEVE190 | - | Infected | 149 | 211 | 10 | 1167 | 7 | 51 | 95 | ND | ND |
| 191GYTVDVKNKRTFLSPWISNI210 | DQ8 | Infected | 216 | 0.2 | 190 | 11 | 231 | 0.5 | 15 | 2488 | >166 |
| 201TFLSPWISNIHEKKGLTKYK220 | DQ8 | - | 3162 | >667 | 1400 | 873 | 327 | 306 | 14 | 3077 | >166 |
| 221SSPEKWSTASDPYSDFEKVT240 | DR4 | - | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 241GRIDKNVSPEARHPLVAAYP260 | DQ8 | Vaccinee, Infected | 2828 | 15 | 55 | 833 | 567 | 9 | 9 | 899 | >166 |
| 261IVHVDMENIILSKNEDQSTQ280 | - | Infected | >6667 | >667 | 167 | >1543 | >1336 | >1908 | >511 | ND | ND |
| 281NTDSETRTISKNTSTSRTHT300 | - | Infected | >6667 | 23 | 179 | 707 | 535 | 60 | >511 | ND | ND |
| 301SEVHGNAEVHASFFDIGGSV320 | DQ8 | Vaccinee, Infected | >6667 | >667 | >1788 | 327 | >1336 | >1908 | >511 | 3 | 7 |
| 321SAGFSNSNSSTVAIDHSLSL340 | DR4 | Infected | 279 | 1 | 6 | 0.4 | 935 | >1908 | 95 | ND | ND |
| 331TVAIDHSLSLAGERTWAETM350 | DR4, DQ8 | Infected | 176 | 0.3 | 10 | 11 | 30 | 4 | 120 | 1056 | 0.1 |
| 361NANIRYVNTGTAPIYNVLPT380 | DR1 | Vaccinee, Infected | 15 | >728 | 0.4 | 1 | 12 | >1288 | 3 | ND | ND |
| 371TAPIYNVLPTTSLVLGKNQT390 | DR1, DR4 | Infected | 1 | 12 | 2 | 0.4 | 78 | 300 | 6 | ND | ND |
| 391LATIKAKENQLSQILAPNNY410 | - | Infected | 89 | 176 | 7 | 179 | 46 | 43 | 0.2 | ND | ND |
| 421LNAQDDFSSTPITMNYNQFL440 | - | Infected | >6667 | >667 | 1265 | 22 | >1336 | >1908 | 77 | ND | ND |
| 431PITMNYNQFLELEKTKQLRL450 | - | Vaccinee, Infected | 15 | 25 | 38 | 1 | 2 | 7 | 0.1 | ND | ND |
| 451DTDQVYGNIATYNFENGRVR470 | DR4 | Infected | 200 | >667 | 293 | 30 | >1336 | >1908 | 2 | ND | ND |
| 461TYNFENGRVRVDTGSNWSEV480 | DR4 | - | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 481LPQIQETTARIIFNGKDLNL500 | DQ8 | Infected | 2 | 5 | 886 | 0.3 | 37 | 2 | 1 | 240 | 6 |
| 491IIFNGKDLNLVERRIAAVNP510 | DQ8 | - | 3801 | 31 | 327 | 267 | 0.1 | 7 | 95 | 693 | 75 |
| 501VERRIAAVNPSDPLETTKPD520 | DQ8 | Vaccinee, Infected | 721 | 75 | 69 | 55 | >1288 | >1288 | 145 | 2506 | 29 |
| 511SDPLETTKPDMTLKEALKIA530 | - | Infected | 211 | 10 | 1800 | 401 | 1000 | 10 | 37 | ND | ND |
| 521MTLKEALKIAFGFNEPNGNL540 | - | Infected | 1155 | 18 | >1788 | 98 | 189 | 10 | 77 | ND | ND |
| 531FGFNEPNGNLQYQGKDITEF550 | - | Infected | >6667 | >667 | 207 | >1543 | 1134 | >1908 | 63 | ND | ND |
| 541QYQGKDITEFDFNFDQQTSQ560 | - | Infected | >2563 | 25 | 306 | >3365 | >1357 | >2733 | 44 | ND | ND |
| 551DFNFDQQTSQNIKNQLAELN570 | - | Infected | 249 | 82 | 239 | 267 | >1336 | 250 | 200 | ND | ND |
| 561NIKNQLAELNATNIYTVLDK580 | DR1 | - | 2 | >728 | 76 | 8 | 137 | 64 | 8 | ND | ND |
| 571ATNIYTVLDKIKLNAKMNIL590 | - | Infected | 31 | 5 | 278 | 19 | 0.5 | 3 | 11 | ND | ND |
| 581IKLNAKMNILIRDKRFHYDR600 | - | Infected | 2160 | 0.1 | 500 | 378 | 6 | 0 | 4 | ND | ND |
| 591IRDKRFHYDRNNIAVGADES610 | DR4, DQ8 | - | 25 | 1 | 0.2 | 4 | 4 | 25 | 2 | 1132 | 0.5 |
| 601NNIAVGADESVVKEAHREVI620 | DR4, DQ8 | - | 4989 | 10 | 1183 | 750 | 732 | 16 | 122 | 2191 | 6 |
| 621NSSTEGLLLNIDKDIRKILS640 | - | Infected | 2236 | 1 | 414 | 80 | 53 | 3 | 77 | ND | ND |
| 631IDKDIRKILSGYIVEIEDTE650 | DR1 | Infected | 5 | 775 | 510 | 1 | 72 | 1026 | 0.3 | ND | ND |
| 641GYIVEIEDTEGLKEVINDRY660 | DR4 | - | 3162 | 82 | 21 | 65 | 433 | 1333 | 0.1 | ND | ND |
| 651GLKEVINDRYDMLNISSLRQ670 | DQ8 | Infected | 7 | 10 | 6 | 22 | 46 | 15 | 6 | >3054 | 1 |
| 661DMLNISSLRQDGKTFIDFKK680 | - | Infected | 200 | 2 | 30 | 27 | 33 | 4 | 27 | ND | ND |
| 671DGKTFIDFKKYNDKLPLYIS690 | - | Vaccinee, Infected | 2494 | 100 | >1788 | 138 | 14 | 25 | 4 | ND | ND |
| 681YNDKLPLYISNPNYKVNVYA700 | DR1, DR4 | Infected | 1 | 4 | 1 | 2 | 2 | 30 | 1 | ND | ND |
| 691NPNYKVNVYAVTKENTIINP710 | - | Infected | 50 | 3 | 75 | 0.3 | 13 | 6 | 10 | ND | ND |
| 711SENGDTSTNGIKKILIFSKK730 | DR4, DQ8 | - | >6667 | >667 | >1788 | 133 | 3 | 217 | 3 | >3054 | >166 |
| 716TSTNGIKKILIFSKKGYEIG735 | DQ8 | - | 183 | 1 | 849 | 35 | 0.4 | 0.4 | 1 | >3054 | >166 |
Binding affinities are expressed as relative values which were calculated as the ratio of the PA peptide IC50 to the IC50 of a reference peptide chosen as a high binder for each allele. Good binding affinity values were interpreted as <100. Means were calculated from at least three independent experiments. ND = Not Done.