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. 2022 Oct 13;9:rbac078. doi: 10.1093/rb/rbac078

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Finding of time dependence of relative viability of cells on PDA-modified surfaces with respect to the virgin substrate as demonstrated by hMSCs on TCP with and without PDA coating. (A) Total viability of hMSCs on modified surfaces during 72 h normalized by those on virgin TCPs at the corresponding time points. PDA-1 and PDA-2 mean the TCPs modified with 1 mg/ml and 2 mg/ml dopamine, respectively. For each group, n = 7. (B) Examination of PDA coating on single cell viability. We measured total DNA contents of hMSCs per well with fluorometric quantification of the DNA-Hoechst 33258 complex reflecting the cell number and the OD values with CCK-8 reflecting the total cell viability. Their combination led to single cell viabilities at each indicated time point, which exhibited no significant difference among time points. Data are presented as mean ± standard deviation and analyzed by one-way ANOVA. For each group, n = 7. Significant differences are marked with one asterisk (P < 0.05) and double asterisks (P < 0.01).