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HOLMES detection platform. The target DNA was specifically amplified by PCR or other amplification methods, and crRNAs were designed to target somewhere in the target DNA. The PAM sequence was designed on the primer, which could be introduced during amplification. The CRISPR/Cas12a-crRNA was then mixed with the amplified product to form a ternary complex if the target DNA was present. The quenched reporter was then cleaved in trans, activating the fluorescence.
