Table 1.

More applications of CRISPR/Cas9 in pathogen detection.

Cas Protein	Pathogen Type	Pathogen	Visualization	Sensitivity	Time	References
Cas9	Viruses	Zika	Colorimetry	1 fM	2–3h	[26]
Cas9	Bacteria	Listeria monocytogenes	LFA	150–200 copies/μL	2 h	[29]
Cas9	Bacteria	E. coli	SDA/RCA	40 CFU/mL	2–3 h	[30]
Cas9n	Bacteria	S. typhimurium	Fluorescence	2 copies/μL	<1 h	[27]
dCas9	Bacteria	Mycobacterium tuberculosis	Bioluminescence	5 × 10−5 nmol/mL	<1 h	[31]
dCas9	Bacteria	Methicillin-resistant Staphylococcus aureus (MRSA)	Fluorescence	10 CFU/mL	<0.5 h	[32]
dCas9	Bacteria	Scrub typhus (ST)/severe fever with thrombocytopenia syndrome (SFTS)	SMR biosensor	0.54 aM/0.63 aM	0.5 h	[33]
dCas9	Viruses	HPV	Microplate reader/eye	-	<0.5 h	[34]
dCas9	Bacteria	Acinetobacter baumannii/Klebsiella pneumoniae	Spectrometry	10−5 mol/L	1 h	[35]