Table 2.

More applications of CRISPR/Cas12a in pathogen detection.

Cas Protein	Pathogen	Platform Name	Amplification Methods	Visualization	Sensitivity	Time	References
LbCas12a	ASFV	POC	RPA/LAMP	Fluorescence	100 fmol	<2 h	[45]
Cas12a	ASFV	LAMP-CRISPR	LAMP	Fluorescence	7 copies/μL	<1 h	[46]
Cas12a	Yersinia pestis	Cas12a-UPTLFA	RPA	UPT-LFA	3 aM	<1 h	[47]
Cas12a	PRRSV	-	RT-RPA	ssDNA-FQ	1 copies/μL	25 min	[48]
Cas12a	Listeria monocytogenes	Cas12aFDet	PCR/RAA	Fluorescence	0.64 aM	15 min	[49]
Cas12a	Vibrio parahaemolyticus	-	RPA	Eye	10−18 M	<30 min	[50]
Cas12a	Toxoplasma gondii	RAA-Cas12a-Tg	RAA	ssDNA-FQ	1 fM	1 h	[51]
Cas12a	Staphylococcus aureus	RAA-Cas12a	RAA	Fluorescence	10 copies/μL	1 h	[52]
Cas12a	pathogenic Yersinia enterocolitica	-	RPA	Eye	1.7 CFU/mL	45 min	[53]
Cas12a	Leptospira	-	RPA	Fluorescence/LFDA	100 cells/mL	<2 h	[54]