Table 3.
More applications of CRISPR/Cas13a in pathogen detection.
| Cas Protein | Pathogen | Platform Name | Amplification Methods |
Visualization | Sensitivity | Time | References |
|---|---|---|---|---|---|---|---|
| LwCas13a | PPRRSV | SHERLOCK | RPA | Eye/LFD | 172 copies/μL | <1 h | [64] |
| LwCas13a | BVDV | SHERLOCK | RT-RPA | Fluorescence | 103 pM | - | [65] |
| LwCas13a | Staphylococcus aureus | CCB-Detection | PCR/T7transcription | Fluorescence | 1 CFU/mL | <4 h | [66] |
| LwCas13a | H7N9 | - | RT-RPA | Fluorescence | 1 fM | 50 min | [67] |
| LwCas13a | Feline calicivirus (FCV) | - | RPA | Fluorescence/LFD | 5.5 copies/μL | - | [68] |
| LwCas13a | TMUV | - | RPA | Fluorescence | 100 copies/μL | 50 min | [69] |
| LwCas13a | ASFV | CRISPR/Cas13a–LFD | RAA | LFD | 10 copies/μL | <2 h | [70] |
| LwCas13a | EMCV | - | RAA | LFD | 10 copies/μL | <1 h | [71] |
| LwCas13a | P. vivax/P. falciparum | SHERLOCK | RPA | Fluorescence | 10 aM | - | [72] |
| LwCas13a | HBV | - | RCA/PCR | Fluorescence | 1 copies/μL | - | [73] |