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. 2022 Oct 18;27(20):6999. doi: 10.3390/molecules27206999

Table 4.

Application of the CRISPR system in pathogen detection.

Cas Protein Detection Platform Guide RNA Target Type Trans-Cleavage
Activity
Amplification Methods Sensitivity
Cas9 sgRNA DNA No CAS-EXPAR aM (10−18)
Cas12 Cas12a HOLMES crRNA DNA Yes PCR/RT-PCR aM
Cas12a DETECTR crRNA DNA Yes RPA aM
Cas12b HOLMESv2 sgRNA DNA Yes LAMP aM
Cas13 Cas13a SHERLOCK crRNA RNA Yes RPA aM
Cas13b SHERLOCKv2 crRNA RNA Yes RPA zM (10−21)
Cas14 Cas14a DETECTR sgRNA ssDNA Yes RPA aM