Table 4.
Cas Protein | Detection Platform | Guide RNA | Target Type | Trans-Cleavage Activity |
Amplification Methods | Sensitivity | |
---|---|---|---|---|---|---|---|
Cas9 | sgRNA | DNA | No | CAS-EXPAR | aM (10−18) | ||
Cas12 | Cas12a | HOLMES | crRNA | DNA | Yes | PCR/RT-PCR | aM |
Cas12a | DETECTR | crRNA | DNA | Yes | RPA | aM | |
Cas12b | HOLMESv2 | sgRNA | DNA | Yes | LAMP | aM | |
Cas13 | Cas13a | SHERLOCK | crRNA | RNA | Yes | RPA | aM |
Cas13b | SHERLOCKv2 | crRNA | RNA | Yes | RPA | zM (10−21) | |
Cas14 | Cas14a | DETECTR | sgRNA | ssDNA | Yes | RPA | aM |