Table 2.
Optimization and colloidal characteristics of blank, LEF EMLs and LEF-SPION EMLs using high-shear homogenization.
| Formulation Code | Homogenization | Particle Size (nm) ± SD | PDI ± SD | Zeta Potential (mV) ± SD | EE % ± SD | |
|---|---|---|---|---|---|---|
| Speed (rpm) | Time (min) | |||||
| F1 | 10,000 | 0 | 416.12 ± 2.3 | 0.256 ± 0.0014 | −23.5 ± 1.3 | ----- |
| F1-LEF | 436.2 ± 1.06 | 0.112 ± 0.004 | −31.5 ± 1.25 | 74.2 ± 0.85 | ||
| F2 | 5 | 398.25 ± 1.45 | 0.15 ± 0.001 | −25.3 ± 2.03 | ----- | |
| F2-LEF | 425.6 ± 1.8 | 0.14 ± 0.0012 | −32.6 ± 2.1 | 75.2 ± 1.04 | ||
| F3 | 10 | 365.3 ± 1.47 | 0.142 ± 0.003 | −22.3 ± 0.98 | ||
| F3-LEF | 385.4± 1.4 | 0.151 ± 0.005 | −24.6 ± 2.05 | 76.4 ± 1.04 | ||
| F4 | 15 | 223.6 ± 1.08 | 0.014 ± 0.012 | −28.3 ± 2.1 | ----- | |
| F4-LEF | 245.3 ± 1.12 | 0.005 ± 0.014 | −31.2 ± 2.23 | 81.5 ± 1.74 | ||
| F5-LEF | 15,000 | 5 | 178.6 ± 1.2 | 0.008 ± 0.0012 | −22.3 ± 1.14 | ----- |
| F5 | 185.6 ± 0.87 | 0.004 ± 0.004 | −25.6 ± 0.65 | 87.3 ± 1.2 | ||
| LEF-SPION F5 | 198.2 ± 1.8 | 0.004 ± 0.001 | −20.5 ± 0.85 | 86.4 ± 1.05 | ||
| SPION | 10.2 ± 2.05 | 0.16 ± 0.0 3 | 23.2 ± 1.22 | ----- | ||