TABLE 2.
MAb reactivities with C-terminal mutants of the PRRSV N protein
| MAb | Fig. 2 panel | Reactivitya with
|
Reference | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PA8 Nb | Rec Nc | C-11d | V112P | R113P | R113D | L114P | I115P | R116P | T118S | P121A | |||
| ISU15A | A | + | + | + | + | + | + | + | + | + | + | + | 28 |
| ISU15B | B | + | + | − | − | − | + | − | − | − | + | + | 28 |
| ISU15C | C | + | + | − | − | − | − | − | − | + | + | − | 28 |
| ISU15E | D | + | + | − | − | − | − | − | − | − | + | + | 28 |
| K9-5 | D | + | + | − | − | − | − | − | − | − | + | + | 29 |
| MR40 | D | + | + | − | − | − | − | − | − | − | + | + | 20 |
| K9-3 | E | + | + | − | − | − | − | − | − | − | − | + | 29 |
| ISU15Fd-11 | F | + | + | − | − | − | − | − | + | − | + | + | 29 |
| ISU15Fd-32 | G | + | + | − | − | − | − | − | +/− | +/− | +/− | + | 29 |
| ISU15Fd | H | + | + | − | − | − | − | − | − | +/− | +/− | + | 29 |
| SDOW17 | I | + | + | − | − | − | +/− | − | +/− | +/− | +/− | + | 19 |
| K9-9 | I | + | + | − | − | − | +/− | − | +/− | +/− | +/− | + | 29 |
| SR30 | J | + | + | − | − | + | + | − | − | − | +/− | + | 20 |
| VO17 | K | + | + | − | + | + | + | − | − | − | + | + | 19 |
| JP25 | L | + | + | − | − | + | + | − | − | − | + | + | 20 |
| EP147 | M | + | + | − | − | − | − | − | − | − | + | − | 19 |
| 1D2 | M | + | + | − | − | − | − | − | − | − | + | − | D. Deregte |
| 7C10 | M | + | + | − | − | − | − | − | − | − | + | − | D. Deregt |
| 2D6 | N | + | + | − | + | − | − | − | − | − | + | +/− | D. Deregt |
| 2G7 | N | + | + | − | + | − | − | − | − | − | + | +/− | D. Deregt |
a
+, positive (N protein detected after 48 h of exposure); +/−, weakly positive (N protein detected after 1 week of exposure); −, negative (N protein undetectable after 1 week of exposure).
b
Authentic N protein from PRRSV-infected cell lysates.
c
Recombinant N protein expressed from vaccinia virus.
d
N protein with a deletion of the 11 C-terminal-most amino acids.
e
Animal Disease Research Institute.