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. 2022 Oct 17;7(10):311. doi: 10.3390/tropicalmed7100311

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The immunoreactivities of line blot assay between the recombinant proteins, rKU_Sej_LRR_2012M and LipL32. The rKU_Sej_LRR_2012M (A) and LipL32 (B) at 50 µg/mL with 9 different rabbit hyperimmune sera against Leptospira serovar Australis (1), Bataviae (2), Canicola (3), Hebdomadis (4), Icterohaemorrhagiae (5), Mini (6), Patoc (7), Pomona (8), Tarassovi (9) and control or non-immunized rabbit serum (10) at dilution 1:100. Goat anti-rabbit IgG conjugated with gold nanoparticle at dilution 1:20 was used as the immunoreactivity color marker.