Table 5.
The validation of peptide-based and LipL32 ELISAs with MAT results from known true positive dogs’ sera.
| Methods | Type of Dogs (No. of Dogs with Positive Results) | ||||
|---|---|---|---|---|---|
| Positive PCR Dogs (6) | Dogs with Positive Results by Peptide-Based and LipL32 ELISAs (5) | Dogs with Negative Results by Peptide-Based and LipL32 ELISAs (5) | Vaccinated Dogs (20) | ||
| MAT (serovar with titer ≥ 1:100) | Autumnalis | 1 | - | - | - |
| Bratislava | - | 1 | - | 8 | |
| Canicola | 4 | 2 | - | 11 | |
| Icterohaemorrhagiae | 3 | 1 | - | 13 * | |
| Pomona | - | - | - | 6 | |
| Ballum | - | 1 | - | - | |
| Sejroe | 5 | 4 | - | - | |
| Tarassovi | 4 | 1 | - | - | |
| Grippotyphosa | - | - | - | 3 | |
| Patoc | 3 | 3 | - | - | |
| Peptide-based ELISAs | 2012-4B | 6 | 5 | 0 | 3 * |
| 2012-5B | 6 | 5 | 0 | 3 * | |
| Protein ELISA | LipL32 | 6 | 5 | 0 | 19 |
* 3 dogs with MAT titer ≥1:400 to serovar Icterohaemorrhagiae.