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. 2022 Oct 21;15(10):1300. doi: 10.3390/ph15101300

Table 2.

Scavenging activity (SC50) of P. spinosa fruit extracts towards multiple oxidants.

NO
(µg/mL)
HOCl
(µg/mL)
O2•−
(µg/mL)
H2O2
(mg/mL)
HO
(mg/mL)
Analytes:
MEF 18.28 ± 0.42 H 40.04 ± 0.51 F 73.89 ± 3.58 D 0.17 ± 0.004 E 0.44 ± 0.02 E,F
MED 43.05 ± 3.08 I 100.90 ± 0.69 G 101.81 ± 3.05 E 0.57 ± 0.007 J 0.81 ± 0.02 H
DEFF 5.61 ± 0.13 F 17.34 ± 0.50 B,C 29.40 ± 0.88 B 0.13 ± 0.002 C 0.33 ± 0.01 D
DEFD 3.85 ± 0.22 D 18.35 ± 0.52 B,C 32.70 ± 2.74 B 0.15 ± 0.002 D 0.32 ± 0.01 D
EAFF 2.15 ± 0.16 C 31.48 ± 0.64 D 45.08 ± 2.96 C 0.14 ± 0.001 C 0.38 ± 0.01 D,E
EAFD 4.79 ± 0.39 E 34.44 ± 0.46 D,E 52.76 ± 2.82 C 0.21 ± 0.005 F 0.33 ± 0.01 D
BFF 6.35 ± 0.41 G 39.78 ± 0.85 F 73.52 ± 2.68 D 0.22 ± 0.002 G 0.47 ± 0.01 F,G
BFD 43.70 ± 2.49 I 96.48 ± 5.41 G 106.88 ± 4.39 E 0.44 ± 0.008 I 0.96 ± 0.06 I
WRF 51.76 ± 3.89 J 38.02 ± 0.88 E,F 103.47 ± 4.06 E 0.27 ± 0.002 H 0.52 ± 0.03 G
WRD 40.21 ± 1.99 I 153.46 ± 0.83 H 311.63 ± 7.08 G 0.72 ± 0.003 K 1.17 ± 0.06 J
Quercetin 0.48 ± 0.03 A 2.08 ± 0.14 A 7.89 ± 0.24 A 0.008 ± 0.001 A 0.05 ± 0.002 A
Cyanidin 3-O-glucoside 1.40 ± 0.09 B 5.62 ± 0.11 A 10.11 ± 0.33 A 0.012 ± 0.001 A,B 0.17 ± 0.002 C
Chlorogenic acid 0.69 ± 0.02 A 13.82 ± 0.53 B 4.58 ± 0.29 A 0.018 ± 0.001 A,B 0.07 ± 0.001 A,B
5-Hydroxymethylfurfural (HMF) ** ** ** *** ***
Reference standards:
Ascorbic acid 0.59 ± 0.04 A 6.32 ± 0.07 A 5.28 ± 0.25 A 0.014 ± 0.001 A,B 0.14 ± 0.005 B,C
Trolox 0.59 ± 0.02 A 21.49 ± 1.21 C 135.33 ± 5.59 F 0.020 ± 0.001 B 0.13 ± 0.003 B,C

SC50, Half scavenging concentration (amount required to reduce the initial oxidant concentration by 50%) calculated in µg/mg of the dry extract or standard/mL of the reaction mixture; ** inactive up to a concentration of 500 µg/mL; *** inactive up to 1500 µg/mL. For extracts codes, see Abbreviations. Data are given as mean of three replicates ± SD. Values in each column labeled with different capital letters (A–K) are significantly different at p < 0.05.