Figure 4.

Representative Western blot and densitometry analysis of the effects of long-term DHA supplementation and/or exercise training on phosphorylated AKT and total AKT (A) and phosphorylated ACC and total ACC (B) in the gastrocnemius muscle of 18-month-old DIO female mice. Band densities of phosphorylated AKT and phosphorylated ACC were normalized by total AKT and total ACC, respectively. Data presented as mean (SD); n = 3–4. The p values for the main factors, DHA supplementation (DHA), exercise (EX) and the interaction between both (DHAxEX) are shown in the corresponding rows of the tables below the figures. Statistically significant p values are marked in bold.