Figure 1.

PCA across different sampling days. Pigs were inoculated intranasally with ASFV/POL/2015/Podlaskie at 0 dpi and euthanised at 6 dpi. Blood samples were collected at 0 dpi (prior to inoculation), at 3 dpi and at 6 dpi (before euthanasia) from each of the 4 pigs. PBMCs were purified from the blood and poly(A)+ mRNA purified and analysed by sequencing (see Materials and Methods). Approximately 27–42 million reads were obtained from each sample and assessed using PCA. The samples from each pig (9–12) on each sampling day are indicated.