Table 1.
Classic CTZ-consuming luciferase BRET systems.
| System | Donor | Acceptor | Substrate | Features | Ref |
|---|---|---|---|---|---|
| BRET1 | Rluc/Rluc8 | EYFP | Coelenterazine-h | [39] | |
| BRET2 | Rluc | GFP2/GFP10 | DeepBlueC (bisdeoxycoelenterazine, coelenterazine 400a, di-dehydro coelenterazine) | Enlarged separation of donor and acceptor emission spectra, higher signal resolution | [40,41] |
| BRET3 | Rluc8 | mOrange | Coelenterazine-h | Longer wavelengths of the emission light and weaker attenuation of biological tissue | [41,45] |
| eBRET | Rluc | eYFP | EnduRen | Prolonged detection timescale from minutes to hours, enhanced luminescence intensity | [42,43,44] |
| BRET3.1 | Rluc8 | mOrange | Coelenterazine-v | [46] | |
| BRET4 | Rluc8 | TagRFP | Coelenterazine-h | [47] | |
| BRET4.1 | Rluc8 | TagRFP | Coelenterazine-v | [46] | |
| BRET5 | Rluc8.6 | TagRFP | Coelenterazine-h | [46] | |
| BRET6 | Rluc8.6 | TurboFP635 | Coelenterazine-h | [46] | |
| BRET6.1 | Rluc8.6 | TurboFP636 | Coelenterazine-v | [46] | |
| BRET7 | Rluc8 | TurboFP637 | Coelenterazine-v | [47] | |
| BRET8 | Rluc8.6 | TurboFP638 | Coelenterazine-h | [47] | |
| BRET9 | ALuc23 | FP, such as mCherry | Coelenterazine | A conceptually unique ligand-activatable BRET system | [48] |