Table 1.
Gene | Forward | Reverse |
---|---|---|
Claudin-3 | 5′-CCC AGC CTA CGG AGT TAC CC-3′ | 5′-TGC CGA TGA ATG CCG AAA CG-3′ |
MFN1 | 5′-AGC TCG CTG TCA TTG GGG AG-3′ | 5′-TCC CTC CAC ACT CAG GAA GC-3′ |
FIS1 | 5′-CTG CGG TGC AGG ATG AAA GAC-3′ | 5′-GGC GTA TTC AAA CTG CGT GCT-3′ |
PGC-1α | 5′-CAG GAG CTG GAT GGC TTG GG-3′ | 5′-GGG CAA AGA GGC TGG TCC T-3′ |
TFAM | 5′ -GCT TCC AGG GGG CTA AGG ATG-3′ | 5′-TCG CCC AAC TTC AGC CAT TT-3′ |
P62 | 5′-CTG AGT CGG CTT CTG CTC CA-3′ | 5′-GCG GCT TCT CTT CCC TCC AT-3′ |
LC3B | 5′-CAT GCC GTC CGA GAA GAC CT-3′ | 5′-CCG GAT GAG CCG GAC ATC TT-3′ |
PINK1 | 5′ -TCG GCC TGT CAG GAG ATC CA-3′ | 5′-CAT TGC AGC CCT TGC CGA TG-3′ |
SOD1 | 5′-AGG GCG TCA TTC ACT TCG AG-3′ | 5′-ACA TGC CTC TCT TCA TCC GCT-3′ |
NF-kB | 5′-CCT CCA CCC CGA CGT ATT GC-3′ | 5′-GCC AAG GCC TGG TTT GAG AT-3′ |
β-actin | 5′-ACA ACC TTC TTG CAG CTC CTC C-3′ | 5′-TGA CCC ATA CCC ACC ATC ACA-3′ |
Abbreviations: MFN1, mitofusin 1; FIS1, fission 1 protein; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1 alpha; TFAM, transcription factor A, mitochondrial; PINK1, (PTEN)-induced putative kinase 1; P62, ubiquitin-binding protein 62; LC3B, microtubule-associated protein 1 A/1B-light chain 3; SOD1, superoxide dismutase 1; NF-kB, nuclear factor kappa-light-chain-enhancer of activated B cells.