Table 1.

Cryo-EM data collection statistics.

Parameter	Cryo-EM Data Collection on D5323–785
Microscope	Technai F30 Polara
High tension (kV)	300
Cs (mm)	2
Detector	Gatan K2 Summit (4k × 4k)
Mode	Counting
Energy filter	No
Magnification	42,000×
Calibrated pixel size	1.21 Å
No. of frames per micrograph	40
Nominal defocus range (µm)	−1.5–3.0
Dose par frame (e·Å−2)	1
No. of micrographs	830