Figure 2.

(A) Binding properties of triblock copolymeric micelles and STAT3-siRNA run on 4% gel agarose electrophoresis. Typical electropherogram (260 nm) of a standard solution containing 2 μM STAT3-siRNA and 4 μg/mL of the internal standard (IS). Percentage of the complexed siRNA versus the N/P ratio obtained by RiboGreen assay (claret) and capillary electrophoresis (purple) (n = 3). (B) Molecular conformation of the complexes by addition of triblock copolymers. Along the filament axis, the angle θ is calculated connecting the nucleotides located at the center of mass and the ending nucleotide bases highlighted in orange. The contribution to the complexation of the polymeric segments PEG, PLL, PLA involved in the complex formation was estimated by calculating the H-bond numbers and the total buried surfaces.