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Stability testing of the multispecies 3D biofilm model (A) with S. aureus, E. coli, and A. baumannii, and the P. aeruginosa 3D biofilm model (B). After 24 h, a stable microbial population is established in both models, which remains stable until 72 h. The ratio of the bacteria to each other in the multispecies 3D biofilm model (A) remains the same during this period.
