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Gradient elution and rate flow used in the chromatographic separation.
| Time (min) | Rate (mL/min) | % A | % B |
|---|---|---|---|
| 0 | 0.200 | 96.0 | 4.0 |
| 2 | 0.200 | 96.0 | 4.0 |
| 7 | 0.200 | 81.7 | 18.3 |
| 12 | 0.223 | 50.0 | 50 |
| 14 | 0.400 | 0.1 | 99.9 |
| 16 | 0.480 | 0.1 | 99.9 |
| 19 | 0.480 | 96.0 | 4.0 |
| 19.10 | 0.200 | 96.0 | 4.0 |
| 20 | 0.200 | 96.0 | 4.0 |
